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Activin Receptor Type IIB antibody [N1C1]

Overexpression
Anti-Activin Receptor Type IIB antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX105330
Anti-Activin Receptor Type IIB antibody [N1C1] used in Western Blot (WB). GTX105330
Anti-Activin Receptor Type IIB antibody [N1C1] used in Western Blot (WB). GTX105330
Anti-Activin Receptor Type IIB antibody [N1C1] used in Western Blot (WB). GTX105330

Cat. No. GTX105330

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Activin Receptor Type IIB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:50000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , SK-N-AS , ACVR2B-transfected 293T , mosue cerebellum

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Activin Receptor Type IIB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036161

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activin A receptor type 2B , ACTRIIB , ActR-IIB , HTX4

Background

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. This gene encodes activin A type IIB receptor, which displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor. [provided by RefSeq]

Database

  • Gene ID: 93 ACVR2B
  • UniProt: Q13705 ACVR2B

Research Area

DATA IMAGES

Anti-Activin Receptor Type IIB antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX105330

GTX105330 IHC-P Image

Activin Receptor Type IIB antibody [N1C1] detects Activin Receptor Type IIB protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
Activin Receptor Type IIB stained by Activin Receptor Type IIB antibody [N1C1] (GTX105330) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Activin Receptor Type IIB antibody [N1C1] used in Western Blot (WB). GTX105330

GTX105330 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Activin Receptor Type IIB antibody [N1C1] (GTX105330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Activin Receptor Type IIB antibody [N1C1] used in Western Blot (WB). GTX105330

GTX105330 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Activin Receptor Type IIB antibody [N1C1] (GTX105330) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Activin Receptor Type IIB antibody [N1C1] used in Western Blot (WB). GTX105330

GTX105330 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Activin Receptor Type IIB antibody [N1C1] (GTX105330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169