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Adenosine A1 Receptor antibody

Anti-Adenosine A1 Receptor antibody used in IHC (Frozen sections) (IHC-Fr). GTX04604
Anti-Adenosine A1 Receptor antibody used in IHC (Frozen sections) (IHC-Fr). GTX04604
Anti-Adenosine A1 Receptor antibody used in Western Blot (WB). GTX04604
Anti-Adenosine A1 Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04604

Cat. No. GTX04604

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
50 μl ($669)
See all Adenosine A1 Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)KKVSASSGDPQKYYGKE, corresponding to amino acid residues 213-229 of human A1AR (Accession P30542).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine A1 receptor , RDC7

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Adenosine A1 Receptor antibody used in IHC (Frozen sections) (IHC-Fr). GTX04604

GTX04604 IHC-Fr Image

IHC-Fr analysis of rat medial septum tissue using GTX04604 Adenosine A1 Receptor antibody.
Panel A : The target signal appears in neurons (right pointing triangles).
Panel B : Parvalbumin staining appears in medial septal neurons.C.
Panel C : Confocal merge of demonstrates expression of A1AR in a subset of medial septal neurons.
Green : Primary antibody
Red : Parvalbumin

Anti-Adenosine A1 Receptor antibody used in IHC (Frozen sections) (IHC-Fr). GTX04604

GTX04604 IHC-Fr Image

IHC-Fr analysis of rat cortex tissue using GTX04604 Adenosine A1 Receptor antibody.
Panel A : The target signal appears in neurons (triangles) and in astrocytes (arrows).
Panel B : Parvalbumin staining appears in cortical interneurons.
Panel C : Confocal merge of images demonstrates the existence of Adenosine A1 Receptor in a subset of cortical interneurons and astrocytes.
Green : Primary antibody
Red : Parvalbumin

Anti-Adenosine A1 Receptor antibody used in Western Blot (WB). GTX04604

GTX04604 WB Image

WB analysis of various tissue lysates using GTX04604 Adenosine A1 Receptor antibody prior incubated with (Lane 3, 4) or without (Lane 1, 2) blocking peptide.
Lane 1 and 3 : Rat brain tissue lysate
Lane 2 and 4 : Rat kidney tissue lysate
Dilution : 1:200

Anti-Adenosine A1 Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04604

GTX04604 ICC/IF Image

ICC/IF analysis PFA-fixed rat dorsal root ganglion cells using GTX04604 Adenosine A1 Receptor antibody.
Orange : Primary antibody
Blue : Hoechst 33342
Dilution : 1:100
Magnification :
Panel A-C : 20x
Panel D-F : 100x

REFERENCE

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Package List Price ($)
$ 669