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Adenosine A2b Receptor antibody

Anti-Adenosine A2b Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX132217
Anti-Adenosine A2b Receptor antibody used in Western Blot (WB). GTX132217
Anti-Adenosine A2b Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX132217
Anti-Adenosine A2b Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX132217
Anti-Adenosine A2b Receptor antibody used in Western Blot (WB). GTX132217
Anti-Adenosine A2b Receptor antibody used in Western Blot (WB). GTX132217
Anti-Adenosine A2b Receptor antibody used in Western Blot (WB). GTX132217

Cat. No. GTX132217

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Adenosine A2b Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human Adenosine A2b Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886590

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine A2b receptor , ADORA2

Cellular Localization

Cell membrane

Background

This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 136 ADORA2B
  • UniProt: P29275 ADORA2B

Research Area

DATA IMAGES

Anti-Adenosine A2b Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX132217

GTX132217 IHC-P Image

Adenosine A2b Receptor antibody detects Adenosine A2b Receptor protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Adenosine A2b Receptor antibody (GTX132217) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Adenosine A2b Receptor antibody used in Western Blot (WB). GTX132217

GTX132217 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine A2b Receptor antibody (GTX132217) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adenosine A2b Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX132217

GTX132217 IHC-P Image

Adenosine A2b Receptor antibody detects Adenosine A2b Receptor protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Adenosine A2b Receptor antibody (GTX132217) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Adenosine A2b Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX132217

GTX132217 IHC-P Image

Adenosine A2b Receptor antibody detects Adenosine A2b Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
Adenosine A2b Receptor stained by Adenosine A2b Receptor antibody (GTX132217) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Adenosine A2b Receptor antibody used in Western Blot (WB). GTX132217

GTX132217 WB Image

PG-4 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine A2b Receptor antibody (GTX132217) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Adenosine A2b Receptor antibody used in Western Blot (WB). GTX132217

GTX132217 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine A2b Receptor antibody (GTX132217) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Adenosine A2b Receptor antibody used in Western Blot (WB). GTX132217

GTX132217 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine A2b Receptor antibody (GTX132217) diluted at 1:1000.

REFERENCE

Application Reference

Wang W et al. J Cell Mol Med 2021; 25 (6) : 2931-2943 Inhibition of adenosine kinase attenuates myocardial ischaemia/reperfusion injury.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169