GeneTex

  • Country / Location Selection

United States (US)

Adenovirus Fiber trimer antibody [2A6]

Cat. No. GTX23232

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2A6

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Adenovirus
Package
500 μl ($399)
See all Adenovirus Fiber products

APPLICATION

Application Note

For IF: Use at an assay dependent dilution. For WB: Use at a dilution of 1:200. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

Adenovirus infected cells and tissues.

Product Note

Recognizes only trimers (high molecular weight bands 180 - 200kDa) on Western blots of unboiled Ad2 and Ad5 fiber proteins and shows immunofluorescence with Ad5 fiber mutant H5ts142 infected cells at 32ºC but not at 39.5ºC.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

UV irradiated Ad2 virus.

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Adenovirus fiber , AD fiber

Cellular Localization

Nuclear

Background

Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta sheet and beta bends, and a knob at the C terminus that contains the type specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the virion.

Research Area

REFERENCE

There are currently no references for Adenovirus Fiber trimer antibody [2A6] (GTX23232). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Adenovirus Fiber trimer antibody [2A6] (GTX23232). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399