Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 |
1:250 |
1:1,000 |
Not tested in other applications.
Calculated MW
Positive Control
MDCK, HepG2, mouse liver, rat brain
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
synthetic peptide corresponding to rat l/s afadin sequence, amino acids 1163-1179, with N-terminal cysteine added, conjugated to BSA.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
afadin, adherens junction formation factor , Af6 , Mllt4
Background
Afadin is a cell-cell adherens junction F-actin binding multidomain protein. Two splicing variants of afadin have been described: l-afadin, which is ubiquitously expressed, and the smaller s-afadin, which is abundantly expressed in neural tissue. l Afadin is a protein containing one PDZ domain in its middle part followed by three proline-rich and one F-actin-binding region at the C-terminal. s Afadin is a protein which lacks both the third proline-rich and the F-actin binding regions and is homologous to the human AF6 (ALL1 fusion partner from chromosome 6) gene product.
Database
Research Area