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Agrin antibody [Agr-131]

Cat. No. GTX12362

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Agr-131

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, RIA

Reactivity

 Mouse, Rat
Package
100 μg ($399)
See all Agrin products

APPLICATION

Application Note

ICC: Use at a dilution of 1/50 - 1/1000. IHC-Fr: Use at an assay dependent dilution. RIA: Use at a concentration of 1 μg/ml. WB: Use at a concentration of 2 μg/ml. Predicted molecular weight: 208.6 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

209 kDa. ( Note )

Product Note

GTX12362 detects all isoforms of agrin. This antibody recognizes all N1 isoforms but, consistent with the epitope mapping investigations, fails to see the N4 protein. Furthermore, the antibody will detect the X, Y and Z splice variants.

PROPERTIES

Form

Liquid

Buffer

50% Glycerol, PBS pH7.2, 0.1mM PMSF

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant rat agrin, C-terminal construct.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

agrin , AGR

Cellular Localization

Cell junction, synapse , Cell membrane

Background

may play a role in synapse development and regeneration [RGD, Feb 2006]

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399