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Ajuba antibody

Anti-Ajuba antibody used in Western Blot (WB). GTX134670
Anti-Ajuba antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134670

Cat. No. GTX134670

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Ajuba products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Ajuba. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887322

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ajuba LIM protein , JUB

Cellular Localization

Cytoplasm, cytoskeleton,Cell membrane,Cell junction,Nucleus

Database

Research Area

DATA IMAGES

Anti-Ajuba antibody used in Western Blot (WB). GTX134670

GTX134670 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ajuba antibody (GTX134670) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ajuba antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134670

GTX134670 ICC/IF Image

Ajuba antibody detects Ajuba protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Ajuba stained by Ajuba antibody (GTX134670) diluted at 1:500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169