APPLICATION
Application Note
Recommended Starting Dilutions: For ELISA: Use at a concentration of 12.5 ng/ml. For WB: Use at a dilution of 1:2,000. Optimal working dilutions should be determined experimentally be the end user.
Calculated MW
Product Note
The Mouse anti-human AR antibody specifically detects targets derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The immunogen corresponding to a region within amino acids 2 and 556 of human Androgen Receptor.
Purification
The IgG fraction of tissue culture supernatant was purified by Protein A/G affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM
Cellular Localization
Nucleus , Cytoplasm
Background
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]
Database
Research Area
DATA IMAGES
![Anti-Androgen Receptor antibody [3C6-F12-F11] used in Western Blot (WB). GTX16032](/templates/default/js/lightbox/images/loading.gif "Anti-Androgen Receptor antibody [3C6-F12-F11] used in Western Blot (WB). GTX16032")
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GTX16032 WB Image
WB analysis of (1) BSA, (2) recombinant protein AR and (3) protein marker using Androgen Receptor antibody [3C6-F12-F11] at a concentration of 0.5 μg/ml.
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REFERENCE
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REVIEW
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![Anti-Androgen Receptor antibody [3C6-F12-F11] used in Western Blot (WB). GTX16032](/upload/website/prouct_img/small/GTX16032/GTX16032_WB_w_23060620_869.webp "Anti-Androgen Receptor antibody [3C6-F12-F11] used in Western Blot (WB). GTX16032")
![Anti-Androgen Receptor antibody [3C6-F12-F11] used in Western Blot (WB). GTX16032](/upload/website/prouct_img/normal/GTX16032/GTX16032_WB_w_23060620_869.webp "Anti-Androgen Receptor antibody [3C6-F12-F11] used in Western Blot (WB). GTX16032")