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Angiopoietin 1 antibody

Orthogonal
Anti-Angiopoietin 1 antibody used in Western Blot (WB). GTX28451
Anti-Angiopoietin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX28451
Anti-Angiopoietin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX28451
Anti-Angiopoietin 1 antibody used in Western Blot (WB). GTX28451

Cat. No. GTX28451

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Angiopoietin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF Assay dependent
IHC-P 1:200-1:800
IP Assay dependent
ELISA 1:5000-1:25000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Product Note

This antibody is specific for mouse Angiopoietin 1 (mAng-1). Partially cross reactivity is noted with human angiopoietin 2.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

80 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide.

Conjugation

Unconjugated

RRID

AB_385958

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiopoietin 1 , 1110046O21Rik , Ang-1 , Ang1

Cellular Localization

Secreted

Background

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognizes a band above 55 kD. This antibody (and the ang-2 antibody, GT28452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.

Database

Research Area

DATA IMAGES

Anti-Angiopoietin 1 antibody used in Western Blot (WB). GTX28451

GTX28451 WB Image

Rabbit anti-Ang-1 (GTX28451) was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 μg of each lysate was used for 10% SDS-PAGE.

Anti-Angiopoietin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX28451

GTX28451 IHC-P Image

IHC-P of ANG1 antibody was diluted 1:500 to detect ANG1 in human lung tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Anti-Angiopoietin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX28451

GTX28451 IHC-P Image

IHC-P analysis of human lung tissue using GTX28451 Angiopoietin 1 antibody.
Dilution : 1:500

Anti-Angiopoietin 1 antibody used in Western Blot (WB). GTX28451

GTX28451 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2019. PMID: 31685802

REFERENCE

Application Reference

Sabirzhanov B et al. Cell Death Dis 2019; 10 (11) : 839 Inhibition of microRNA-711 limits angiopoietin-1 and Akt changes, tissue damage, and motor dysfunction after contusive spinal cord injury in mice.
Application : WB
Reactivity : Mouse
Chiang WC et al. Mol Med 2019; 25 (1) : 5 Angiopoietin 1 influences ischemic reperfusion renal injury via modulating endothelium survival and regeneration.
Application : IHC-P
Reactivity : Mouse
Sabirzhanov B et al. J Neurotrauma 2018; (Epub) miR-711 induced down-regulation of Angiopoietin-1 mediates neuronal cell death.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399