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Apolipoprotein D antibody

Anti-Apolipoprotein D antibody used in IHC (Paraffin sections) (IHC-P). GTX101306
Anti-Apolipoprotein D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101306
Anti-Apolipoprotein D antibody used in IHC (Paraffin sections) (IHC-P). GTX101306
Anti-Apolipoprotein D antibody used in Western Blot (WB). GTX101306

Cat. No. GTX101306

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Apolipoprotein D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Apolipoprotein D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036251

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein D

Cellular Localization

Secreted

Background

This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein D antibody used in IHC (Paraffin sections) (IHC-P). GTX101306

GTX101306 IHC-P Image

Apolipoprotein D antibody detects Apolipoprotein D protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Apolipoprotein D antibody (GTX101306) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Apolipoprotein D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101306

GTX101306 ICC/IF Image

Apolipoprotein D antibody detects Apolipoprotein D protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein D protein stained by Apolipoprotein D antibody (GTX101306) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Apolipoprotein D antibody used in IHC (Paraffin sections) (IHC-P). GTX101306

GTX101306 IHC-P Image

Apolipoprotein D antibody detects Apolipoprotein D protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Apolipoprotein D stained by Apolipoprotein D antibody (GTX101306) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Apolipoprotein D antibody used in Western Blot (WB). GTX101306

GTX101306 WB Image

Apolipoprotein D antibody detects Apolipoprotein D protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein D antibody (GTX101306) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169