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Asparagine synthetase antibody

Anti-Asparagine synthetase antibody used in Western Blot (WB). GTX00925
Anti-Asparagine synthetase antibody used in Western Blot (WB). GTX00925
Anti-Asparagine synthetase antibody used in Western Blot (WB). GTX00925
Anti-Asparagine synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00925

Cat. No. GTX00925

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Arabidopsis thaliana, Plant
Package
200 μg ($419)
See all Asparagine synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
IHC-P 1:100-1:500
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Arabidopsis thaliana

Immunogen

Purified recombinant Arabidopsis asparagine synthetase isoprotein 2 (ASN2 ), full-size, no-tag attached

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-Asparagine synthetase antibody used in Western Blot (WB). GTX00925

GTX00925 WB Image

WB analysis of various arabidopsis leaf extract using GTX00925 Asparagine synthetase antibody.
Lane 1 : Col0 is wild-type Arabidopsis plant
Lane 2 & 3 : asn2-1 and asn2-2 are T1 insertion mutants, respectively.

Anti-Asparagine synthetase antibody used in Western Blot (WB). GTX00925

GTX00925 WB Image

WB analysis of absence of ASN1 protein in extracts of floral organ of asn1 insertion mutant using GTX00925 Asparagine synthetase antibody. Wild type (Col-0) samples are analyzed in the upper window, and asn1 mutant samples are in the lower window. The upper numerals are the protein levels measured by densitometric tracing of western blot. Developmental stages are indicated below. Marker proteins are 72 and 55 kDa.

Anti-Asparagine synthetase antibody used in Western Blot (WB). GTX00925

GTX00925 WB Image

WB analysis of Arabidopsis leaf extract using GTX00925 Asparagine synthetase antibody. Molecular mass of Arabidopsis ASN2 is 65 kDa.
Dilution : 1:1000
Loading : 10 μg

Anti-Asparagine synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00925

GTX00925 ICC/IF Image

ICC/IF analysis of Arabidopsis thin leaf section using GTX00925 Asparagine synthetase antibody.
Blue: Primary antibod
Red: Autofluorescence

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 419