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Aspartoacylase antibody

Anti-Aspartoacylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110699
Anti-Aspartoacylase antibody used in Western Blot (WB). GTX110699
Anti-Aspartoacylase antibody used in Western Blot (WB). GTX110699

Cat. No. GTX110699

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Aspartoacylase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Mouse brain , ASPA-transfected 293T

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Aspartoacylase Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036282

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aspartoacylase , ACY2 , ASP

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Aspartoacylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110699

GTX110699 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Aspartoacylase(GTX110699) antibody at 1:500 dilution.

Anti-Aspartoacylase antibody used in Western Blot (WB). GTX110699

GTX110699 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aspartoacylase antibody (GTX110699) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Aspartoacylase antibody used in Western Blot (WB). GTX110699

GTX110699 WB Image

Sample (20 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX110699 diluted at 1:10000

REFERENCE

Application Reference

Roth LM et al. Glia 2021; 69 (2) : 362-376 Differential effects of integrase strand transfer inhibitors, elvitegravir and raltegravir, on oligodendrocyte maturation: A role for the integrated stress response.
Application : IHC-Fr
Reactivity : Mouse
Long PM et al. J Biol Chem 2013; 288 (36) : 26188-200 N-acetylaspartate (NAA) and N-acetylaspartylglutamate (NAAG) promote growth and inhibit differentiation of glioma stem-like cells.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169