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Avidin antibody (HRP)

Anti-Avidin antibody (HRP) used in Western Blot (WB). GTX27233

Cat. No. GTX27233

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, Dot, ELISA, IHC

Reactivity

Chicken
Package
1 mg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000-1:5000
Dot Assay dependent
ELISA 1:10000-1:50000
IHC 1:500-1:2000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Product Note

No cross reactivity occurs against Streptavidin.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

Avidin collected from hen egg white

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

avidin

Cellular Localization

Secreted

Background

The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).

Database

Research Area

DATA IMAGES

Anti-Avidin antibody (HRP) used in Western Blot (WB). GTX27233

GTX27233 WB Image

WB analysis of various samples using GTX27233 Avidin antibody (HRP).
Lane 1 : Protein ladder
Lane 2 : Avidin
Lane 3 : Streptavidin
Loading : 0.05 μg
Dilution : 1 μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 399