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B-Raf (V600E Mutant Specific) antibody [RM8]

Orthogonal
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in Western Blot (WB). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

Cat. No. GTX33595

Reference 3

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM8

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($649)
See all B-Raf products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
ICC/IF 0.5 - 5 μg/mL
IHC-P 0.5 - 5 μg/mL
ELISA 0.5 - 2 μg/mL
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Product Note

This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to BRAF V600E mutant

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2887722

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRAF,BRAF1,BRaf protooncogene, serine/threonine kinase,Braf,NS7,RAFB1,B-Raf,B-Raf proto-oncogene, serine/threonine kinase

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in Western Blot (WB). GTX33595

GTX33595 WB Image

Orthogonal

WB analysis of cells expressing endogenous mutant (V600E) BRAF or wild type (WT) protein using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of melanoma tissue using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of formalin fixed and paraffin embedded sections of SW40 cells (B-Raf Wide type) using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of thyroid Carcinoma tissue using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of formalin fixed and paraffin embedded sections of WiDr cells (B-Raf V600E mutant) using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of thyroid Carcinoma tissue using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

REFERENCE

Application Reference

CP Chen et al. Heliyon 2024; 10 (7) : 29055 Synergistic effects of the combination of trametinib and alpelisib in anaplastic thyroid cancer with BRAF and PI3KCA co-mutations
Application : WB
Makboul R et al. Nucl Med Commun 2020; 41 (5) : 416-425 Do BRAFV600E mutation and sodium-iodide symporter expression affect the response to radioactive iodine therapy in patients with papillary thyroid carcinoma?
Application : IHC-P
Reactivity : Human
Hansen CH et al. Proc Natl Acad Sci U S A 2017; (Epub) Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection.
Application : ELISA
Submit a Reference

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Package List Price ($)
$ 649