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B-Raf antibody [1H12]

Anti-B-Raf antibody [1H12] used in IHC (Paraffin sections) (IHC-P). GTX83134
Anti-B-Raf antibody [1H12] used in Western Blot (WB). GTX83134
Anti-B-Raf antibody [1H12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83134

Cat. No. GTX83134

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H12

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all B-Raf products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human BRAF expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-B-Raf antibody [1H12] used in IHC (Paraffin sections) (IHC-P). GTX83134

GTX83134 IHC-P Image

IHC-P analysis of human testis tissue using GTX83134 B-Raf antibody [1H12].

Anti-B-Raf antibody [1H12] used in Western Blot (WB). GTX83134

GTX83134 WB Image

WB analysis of HeLa (1), HL60 (2), HepG2 (3) and NIH3T3 (4) cell lysate using GTX83134 B-Raf antibody [1H12].

Anti-B-Raf antibody [1H12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83134

GTX83134 ICC/IF Image

ICC/IF analysis of MCF-7 (left) and HepG2 (right) cells using GTX83134 B-Raf antibody [1H12].
Green : B-Raf
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399