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B7-H4 antibody [B7H4/2652R]

Anti-B7-H4 antibody [B7H4/2652R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02741
Anti-B7-H4 antibody [B7H4/2652R] used in IHC (Paraffin sections) (IHC-P). GTX02741
Anti-B7-H4 antibody [B7H4/2652R] used in Flow cytometry (FACS). GTX02741

Cat. No. GTX02741

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 B7H4/2652R

Isotype

 IgG

Application

 ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($529)
See all B7-H4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2 μg/ml
IHC-P 1-2 μg/ml
FACS 1-2μg/million cells in 0.1ml
ELISA 2-4 μg/ml (for coating)

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A recombinant fragment of human B7-H4 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

V-set domain containing T cell activation inhibitor 1 , B7-H4 , B7H4 , B7S1 , B7X , B7h.5 , PRO1291 , VCTN1

Cellular Localization

Cell membrane

Background

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-B7-H4 antibody [B7H4/2652R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02741

GTX02741 ICC/IF Image

ICC/IF analysis of SKBR-3 cells using GTX02741 B7-H4 antibody [B7H4/2652R].
Green : Primary antibody
Red : nuclear counterstain

Anti-B7-H4 antibody [B7H4/2652R] used in IHC (Paraffin sections) (IHC-P). GTX02741

GTX02741 IHC-P Image

IHC-P analysis of human testicular carcinoma section using GTX02741 B7-H4 antibody [B7H4/2652R].

Anti-B7-H4 antibody [B7H4/2652R] used in Flow cytometry (FACS). GTX02741

GTX02741 FACS Image

FACS analysis of SKBR-3 cells using GTX02741 B7-H4 antibody [B7H4/2652R].
Blue : Primary antibody
Red : Isotype control

REFERENCE

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REVIEW

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Package List Price ($)
$ 529