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BAF53B antibody

Anti-BAF53B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65855
Anti-BAF53B antibody used in IHC (Paraffin sections) (IHC-P). GTX65855
Anti-BAF53B antibody used in Western Blot (WB). GTX65855
Anti-BAF53B antibody used in IHC (Paraffin sections) (IHC-P). GTX65855
Anti-BAF53B antibody used in IHC (Paraffin sections) (IHC-P). GTX65855

Cat. No. GTX65855

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all BAF53B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-280 of human ACTL6B (NP_057272.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin like 6B , ACTL6 , BAF53B , arpNalpha

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-BAF53B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65855

GTX65855 ICC/IF Image

ICC/IF analysis of A549 cells using GTX65855 BAF53B antibody.

Anti-BAF53B antibody used in IHC (Paraffin sections) (IHC-P). GTX65855

GTX65855 IHC-P Image

IHC-P analysis of human kidney tissue using GTX65855 BAF53B antibody.
Dilution : 1:100

Anti-BAF53B antibody used in Western Blot (WB). GTX65855

GTX65855 WB Image

WB analysis of various sample lysates using GTX65855 BAF53B antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-BAF53B antibody used in IHC (Paraffin sections) (IHC-P). GTX65855

GTX65855 IHC-P Image

IHC-P analysis of mouse jejunum tissue using GTX65855 BAF53B antibody.
Dilution : 1:100

Anti-BAF53B antibody used in IHC (Paraffin sections) (IHC-P). GTX65855

GTX65855 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX65855 BAF53B antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399