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BHMT antibody

Anti-BHMT antibody used in Western Blot (WB). GTX103037
Anti-BHMT antibody used in IHC (Paraffin sections) (IHC-P). GTX103037
Anti-BHMT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103037
Anti-BHMT antibody used in IHC (Paraffin sections) (IHC-P). GTX103037
Anti-BHMT antibody used in Western Blot (WB). GTX103037
Anti-BHMT antibody used in Western Blot (WB). GTX103037

Cat. No. GTX103037

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all BHMT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HepG2 , mouse liver

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BHMT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949742

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

betaine--homocysteine S-methyltransferase , BHMT1 , HEL-S-61p

Cellular Localization

Cytoplasm

Background

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BHMT antibody used in Western Blot (WB). GTX103037

GTX103037 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
10% SDS PAGE
GTX103037 diluted at 1:1000

Anti-BHMT antibody used in IHC (Paraffin sections) (IHC-P). GTX103037

GTX103037 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using BHMT antibody (GTX103037) at 1:300 dilution.

Anti-BHMT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103037

GTX103037 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using BHMT(GTX103037) antibody at 1:500 dilution.

Anti-BHMT antibody used in IHC (Paraffin sections) (IHC-P). GTX103037

GTX103037 IHC-P Image

BHMT antibody detects BHMT protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
BHMT antibody (GTX103037) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BHMT antibody used in Western Blot (WB). GTX103037

GTX103037 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
10% SDS PAGE
GTX103037 diluted at 1:1000

Anti-BHMT antibody used in Western Blot (WB). GTX103037

GTX103037 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BHMT antibody (GTX103037) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 18498552

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169