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BICD2 antibody

KO/KD
Anti-BICD2 antibody used in Western Blot (WB). GTX120683
Anti-BICD2 antibody used in Western Blot (WB). GTX120683
Anti-BICD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX120683
Anti-BICD2 antibody used in Western Blot (WB). GTX120683

Cat. No. GTX120683

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BICD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , PC-12 , Rat2 , 293T

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BICD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885513

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BICD cargo adaptor 2 , SMALED2 , SMALED2A , SMALED2B , bA526D8.1

Cellular Localization

Golgi apparatus , Cytoplasm , cytoskeleton

Background

This gene is one of two human homologs of Drosophila bicaudal-D and a member of the Bicoid family. It has been implicated in dynein-mediated, minus end-directed motility along microtubules. It has also been reported to be a phosphorylation target of NIMA related kinase 8. Two alternative splice variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BICD2 antibody used in Western Blot (WB). GTX120683

GTX120683 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody (GTX120683) diluted at 1:1000.

Anti-BICD2 antibody used in Western Blot (WB). GTX120683

GTX120683 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody (GTX120683) diluted at 1:1000.

Anti-BICD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX120683

GTX120683 IHC-P Image

BICD2 antibody detects BICD2 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
BICD2 antibody (GTX120683) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BICD2 antibody used in Western Blot (WB). GTX120683

GTX120683 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody (GTX120683) diluted at 1:1000.

REFERENCE

Application Reference

Gon?alves JC et al. Curr Biol 2020; 30 (16) : 3116-3129.e4 Nesprin-2 Recruitment of BicD2 to the Nuclear Envelope Controls Dynein/Kinesin-Mediated Neuronal Migration In?Vivo.
Application : IHC
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169