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BMP4 antibody

Orthogonal
Anti-BMP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX100874
Anti-BMP4 antibody used in Western Blot (WB). GTX100874
Anti-BMP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100874

Cat. No. GTX100874

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all BMP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HepG2 conditioned medium

Product Note

This antibody is able to recognize full length BMP4 protein (~55kDa) and N-terminal propeptide BMP4 protein (~40kDa) but unable to detect mature form.

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BMP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949749

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bone morphogenetic protein 4 , BMP2B , BMP2B1 , MCOPS6 , OFC11 , ZYME

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BMP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX100874

GTX100874 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using BMP4(GTX100874) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BMP4 antibody used in Western Blot (WB). GTX100874

GTX100874 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100874) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BMP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100874

GTX100874 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using BMP4(GTX100874) antibody at 1:100 dilution.

REFERENCE

Application Reference

Kathleen M McAndrews et al. Oncogene 2021; 40 (26) : 4440-4452 αSMA+ fibroblasts suppress Lgr5+ cancer stem cells and restrain colorectal cancer progression.
Application : IHC-P
Liqin An et al. Genes Dis 2021; 8 (4) : 531-544 Bone morphogenetic protein 4 (BMP4) promotes hepatic glycogen accumulation and reduces glucose level in hepatocytes through mTORC2 signaling pathway.
Guo L et al. Sci Rep 2019; 9 (1) : 2867 TSA restores hair follicle-inductive capacity of skin-derived precursors.
Peng Q et al. Aging (Albany NY) 2019; 11 (23) : 11520-11540 Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes.
Application : WB
Reactivity : Human
Chiou J et al. Sci Rep 2017; 7 (1) : 9830 Decrease of FSTL1-BMP4-Smad signaling predicts poor prognosis in lung adenocarcinoma but not in squamous cell carcinoma.
Application : IHC-P
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169