GeneTex
United States (US)

BNP antibody [24C5] (HRP)

BNP-antibody-24C5-HRP_Western blot_GTX14699-2_18121409_480.jpg
BNP-antibody-24C5-HRP_Western blot_GTX14699-3_18121409_624.jpg
BNP-antibody-24C5-HRP_Western blot_GTX14699-1_18121409_984.jpg

Cat No. GTX14699

Host Mouse
Clonality Monoclonal
Clone Name 24C5
Isotype IgG1
Application WB, ELISA, EIA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
EIA Assay dependent
Not tested in other applications.

Calculated MW

14.73 kDa. ( Note )

Specificity/Sensitivity

Human BNP and proBNP.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic BNP peptide 11-FGRKMDRISSSS-22 conjugated with carrier protein.

Purification

Protein A purified

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Natriuretic Peptide B,Bnp,Nppb

Cellular Localization

Secreted

Background

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES
BNP-antibody-24C5-HRP_Western blot_GTX14699-2_18121409_480.jpg

GTX14699 WB Image

Immunodetection of recombinant proBNP (E. coli)in Western blotting by BNP antibody [24C5] after Tricine-SDS-PAGE under reducing conditions

BNP-antibody-24C5-HRP_Western blot_GTX14699-3_18121409_624.jpg

GTX14699 WB Image

Immunodetection of recombinant proBNP (mammalian cell line)in Western blotting by BNP antibody [24C5] after Tricine-SDS-PAGE under reducing conditions

BNP-antibody-24C5-HRP_Western blot_GTX14699-1_18121409_984.jpg

GTX14699 WB Image

Immunodetection of human synthetic BNP in Western blotting by BNP antibody [24C5] after Tricine-SDS-PAGE under reducing conditions