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BRCA1 antibody

Anti-BRCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50692
Anti-BRCA1 antibody used in Western Blot (WB). GTX50692

Cat. No. GTX50692

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Potorous tridactylus/Potoroo
Package
100 μl ($399)
See all BRCA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

208 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.1522~1526 (Y-P-S-Q-E) derived from human BRCA1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170530

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-BRCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50692

GTX50692 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50692 BRCA1 antibody.

Anti-BRCA1 antibody used in Western Blot (WB). GTX50692

GTX50692 WB Image

WB analysis of extracts from 293 and MCF cells using GTX50692 BRCA1 antibody.

REFERENCE

Application Reference

Alcaraz Silva B et al. J Biol Chem 2014; DNA Damage to a single chromosome end delays anaphase onset.
Application : ICC/IF
Reactivity : Potorous tridactylus/Potoroo
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REVIEW

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Application Reference
Alcaraz Silva B et al. J Biol Chem 2014; DNA Damage to a single chromosome end delays anaphase onset.
Application : ICC/IF
Reactivity : Potorous tridactylus/Potoroo
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399