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BRP44L antibody

Anti-BRP44L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87153
Anti-BRP44L antibody used in IHC (Paraffin sections) (IHC-P). GTX87153
Anti-BRP44L antibody used in Western Blot (WB). GTX87153

Cat. No. GTX87153

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all BRP44L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human BRP44L (11-60).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10730773

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BRP44L , CGI129 , MPC1 , MPYCD , dJ68L15.3 , mitochondrial pyruvate carrier 1

Cellular Localization

Mitochondrion inner membrane

Background

The protein encoded by this gene is part of an MPC1/MPC2 heterodimer that is responsible for transporting pyruvate into mitochondria. The encoded protein is found in the inner mitochondrial membrane. Defects in this gene are a cause of mitochondrial pyruvate carrier deficiency. Several transcript variants, some protein coding and one non-protein coding, have been found for this gene. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-BRP44L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87153

GTX87153 ICC/IF Image

ICC/IF analysis of A549 cells using GTX87153 BRP44L antibody. The picture on the right is blocked with the synthesized peptide.

Anti-BRP44L antibody used in IHC (Paraffin sections) (IHC-P). GTX87153

GTX87153 IHC-P Image

IHC-P analysis of human heart tissue using GTX87153 BRP44L antibody. The picture on the right is blocked with the synthesized peptide.

Anti-BRP44L antibody used in Western Blot (WB). GTX87153

GTX87153 WB Image

WB analysis of COLO and HeLa cell lysates using GTX87153 BRP44L antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Chen CN et al. Am J Physiol Endocrinol Metab. 2015; 308 (11) : 942-9 Late-onset caloric restriction alters skeletal muscle metabolism by modulating pyruvate metabolism.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399