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BRRN1 antibody

Anti-BRRN1 antibody used in Western Blot (WB). GTX64648
Anti-BRRN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64648

Cat. No. GTX64648

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all BRRN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:5000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NCAPH (NP_056156.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

non-SMC condensin I complex subunit H , BRRN1 , CAP-H , CAPH , MCPH23

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-BRRN1 antibody used in Western Blot (WB). GTX64648

GTX64648 WB Image

WB analysis of various sample lysates using GTX64648 BRRN1 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-BRRN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64648

GTX64648 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64648 BRRN1 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399