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BUB3 antibody

Anti-BUB3 antibody used in Western Blot (WB). GTX31314
Anti-BUB3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31314
Anti-BUB3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31314

Cat. No. GTX31314

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all BUB3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bub3 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human bub3.The immunogen is located within the last 50 amino acids of Bub3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BUB3 mitotic checkpoint protein , BUB3L , hBUB3

Cellular Localization

Nucleus

Background

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BUB3 antibody used in Western Blot (WB). GTX31314

GTX31314 WB Image

WB analysis of Jurkat cell lysate using GTX31314 BUB3 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-BUB3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31314

GTX31314 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31314 BUB3 antibody.
Working concentration : 20 μg/ml

Anti-BUB3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31314

GTX31314 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31314 BUB3 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399