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Bacillus anthracis protective antigen antibody

Anti-Bacillus anthracis protective antigen antibody used in Western Blot (WB). GTX13809
Anti-Bacillus anthracis protective antigen antibody used in Western Blot (WB). GTX13809

Cat. No. GTX13809

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Bacillus anthracis
Package
200 μl ($399)
See all Bacillus anthracis protective antigen products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml - 10 μg/ml
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Serum diluted with PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Bacillus anthracis

Immunogen

The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 186-192 of the Anthrax PA protein (approx. 83 kDa).

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63 kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family.

Research Area

DATA IMAGES

Anti-Bacillus anthracis protective antigen antibody used in Western Blot (WB). GTX13809

GTX13809 WB Image

WB analysis of recombinant anthrax PA protein using GTX13809 Bacillus anthracis protective antigen antibody.
Lane 1 : pre-bleed
Lane 2 : primary antibody
Dilution : 1:1000

Anti-Bacillus anthracis protective antigen antibody used in Western Blot (WB). GTX13809

GTX13809 WB Image

WB analysis of recombinant anthrax PA protein using GTX13809 Bacillus anthracis protective antigen antibody.
Lane 1 : pre-bleed
Lane 2 : primary antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399