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Basophils antibody [2D7]

Cat. No. GTX54490

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2D7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human
Package
50 μg ($399)
See all Basophils products
PRODUCT

Summary

The 2D7 antibody can be used as a sensitive and precise marker for human basophils- and does not react with lymphocytes, monocytes, eosinophils, neutrophils- or mast cells in immunohistochemistry.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent

Note :

WB
A reduced sample treatment and SDS-Page was used.

ICC/IF
Cytospin preparations were fixed in methanol for 15 min, incubated in methanol containing 0.6% H2O2, for 30 min to block endogenous peroxidase, washed in dH2O, incubated with goat serum (1/500 dilution) for 2 h to block nonspecific staining. Blocked slides were washed with TTBS, pH7.4.

IHC-P
Fresh surgical tissues were fixed in Carnoy's fluid for 24 h and transferred to absolute ethanol. Tissues were embedded in paraffin, and 4- μm sections were prepared. Tissue sections were dewaxed in xylene and rehydrated in graded ethanol solutions. Endogenous peroxidase was inhibited.

IHC-Fr
Sections were fixed in either 10% NBF or Carnoy's fluid for 15 minutes at RT. NBF-fixed tissue sections were digested with 0.1% protease in TTBS for 20 min at RT. Endogenous peroxidase activity was inhibited by methanol with 0.6% H2O2 for 30 min at RT. Blocked by incubation in horse serum at 1:20 dilution in TTBS for 1 hr at RT. 2D7 at a 1:300 dilution in TTBS overnight at 4 ° C or with a murine mAb of undetermined specificity (MOPC31-C) as a negative control.
Not tested in other applications.

Product Note

This antibody stains the extracellular domain of basophils.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Lysate of purified basophils

Purification

Purified IgG1

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 399