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Bif-1 antibody, C-term

Anti-Bif-1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89961
Anti-Bif-1 antibody, C-term used in Western Blot (WB). GTX89961

Cat. No. GTX89961

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Bif-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 2.5μg/ml
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89961-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QKGKVPITYLELLN, from the C Terminus of the protein sequence according to NP_057093.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10732081

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SH3 domain containing GRB2 like, endophilin B1 , Bif-1 , CGI-61 , PPP1R70 , dJ612B15.2

Cellular Localization

Cytoplasm , Golgi apparatus membrane , Mitochondrion outer membrane

Background

This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-Bif-1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89961

GTX89961 IHC-P Image

IHC-P analysis of human colon using GTX89961 Bif-1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-Bif-1 antibody, C-term used in Western Blot (WB). GTX89961

GTX89961 WB Image

WB analysis of Jurkat lysate using GTX89961 Bif-1 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Serfass JM et al. J Biol Chem 2017; 292 (24) : 10097-10111 Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399