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Brachyury antibody [HL2285]

Recombinant antibody
Anti-Brachyury antibody [HL2285] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638338

Cat. No. GTX638338

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2285

Isotype

 IgG

Application

 ICC/IF

Reactivity

 Rat
Package
100 μl ($399),
25 μl ($169)
See all Brachyury products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Brachyury.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

T-box transcription factor T , SAVA , T , TFT

Cellular Localization

Nucleus

Background

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. [provided by RefSeq, Sep 2018]

Database

Research Area

DATA IMAGES

Anti-Brachyury antibody [HL2285] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638338

GTX638338 ICC/IF Image

Brachyury antibody [HL2285] detects Brachyury protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Brachyury stained by Brachyury antibody [HL2285] (GTX638338) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations