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Brachyury antibody

Comparison
Anti-Brachyury antibody used in IHC (Paraffin sections) (IHC-P). GTX133714
Anti-Brachyury antibody used in Western Blot (WB). GTX133714
Anti-Brachyury antibody used in IHC (Paraffin sections) (IHC-P). GTX133714

Cat. No. GTX133714

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all Brachyury products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

mouse testis

Predict Reactivity

Human, Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Brachyury. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887073

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

T-box transcription factor T , SAVA , T , TFT

Cellular Localization

Nucleus

Background

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Brachyury antibody used in IHC (Paraffin sections) (IHC-P). GTX133714

GTX133714 IHC-P Image

Brachyury antibody detects Brachyury protein at nucleus in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Brachyury antibody (GTX133714) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Brachyury antibody used in Western Blot (WB). GTX133714

GTX133714 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Brachyury antibody (GTX133714) diluted at 1:1000 and competitor's antibody (sc-17743) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Brachyury antibody used in IHC (Paraffin sections) (IHC-P). GTX133714

GTX133714 IHC-P Image

Brachyury antibody detects Brachyury protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 mouse embryo.
Green: Brachyury stained by Brachyury antibody (GTX133714) diluted at 1:250.
Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (GTX627405) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169