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C1q antibody [JL-1]

Cat. No. GTX54404

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 JL-1

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-Fr, ELISA, Depletion, PLA

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all C1q products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
ELISA Assay dependent
Depletion Assay dependent
PLA Assay dependent

Note :

IHC-Fr
Antibody JL-1 was used to stain tissue sections which were fixed in acetone.

Depletion
This antibody was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice.
Not tested in other applications.

Product Note

The monoclonal antibody JL-1 is reactive with the collagen-like region (CLR) only, which is the same region to which autoantibodies in mice and humans are binding. Anti-C1q autoantibodies deposit in glomeruli together with C1q but induce overt renal disease only in the context of glomerular immune complex disease. This provides an explanation why anti-C1q antibodies are especially pathogenic in patients with SLE.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Purified mouse C1q

Purification

Protein G purified

Endotoxin

< 24 EU/mg (Determined by LAL assay)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

complement component 1, q subcomponent, alpha polypeptide , complement component 1, q subcomponent, beta polypeptide , complement component 1, q subcomponent, C chain , AI255395 , Adic , C1q , Adia , AI385742 , Adib , C1qg , Ciqc

Cellular Localization

Secreted

Database

Research Area

REFERENCE

Application Reference

Xu F et al. BMC Med 2023; 21 (1) : 7 Prolonged anesthesia induces neuroinflammation and complement-mediated microglial synaptic elimination involved in neurocognitive dysfunction and anxiety-like behaviors.
Vad?szi H et al. J Biol Chem 2022; 298 (7) : 102113 Competitive inhibition of the classical complement pathway using exogenous single-chain C1q recognition proteins.

Clone Reference

F Benavente et al. et al. Elife 2020; Novel C1q receptor-mediated signaling controls neural stem cell behavior and neurorepair
Application : PLA
Reactivity : Human
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Package List Price ($)
$ 399