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C3G antibody

Anti-C3G antibody used in IHC (Paraffin sections) (IHC-P). GTX133726
Anti-C3G antibody used in Western Blot (WB). GTX133726
Anti-C3G antibody used in IHC (Paraffin sections) (IHC-P). GTX133726

Cat. No. GTX133726

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all C3G products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human C3G. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887081

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rap guanine nucleotide exchange factor 1 , C3G , GRF2

Background

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C3G antibody used in IHC (Paraffin sections) (IHC-P). GTX133726

GTX133726 IHC-P Image

C3G antibody detects C3G protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
C3G stained by C3G antibody (GTX133726) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C3G antibody used in Western Blot (WB). GTX133726

GTX133726 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with C3G antibody (GTX133726) diluted at 1:1000.

Anti-C3G antibody used in IHC (Paraffin sections) (IHC-P). GTX133726

GTX133726 IHC-P Image

C3G antibody detects C3G protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
C3G stained by C3G antibody (GTX133726) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169