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C5b-9 antibody [2A1]

Cat. No. GTX54500

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2A1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

 Rat
Package
50 μg ($399)
See all C5b-9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
ELISA Assay dependent

Note :

WB
A non-reduced sample treatment and SDS-Page was used.

IHC-P
Tissue sections fixed in formalin were pretreated with protease type XXIV for 10 minutes at 37⁰C before incubation.

IHC-Fr
Tissue sections were fixed in acetone for 10 minutes at room temperature before incubation.

ELISA
Plates were coated with 30μg/ml in 50mM carbonatebuffer, pH10.6 for 16h at 4ºC.
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Product Note

The antibody was shown to compete with antibodies to human C9 for its binding site on the C5b-9 complex, indicating that the reactive epitope is located on the C9 molecule. Monoclonal antibody 2A1 can be used as a coating antibody to detect C5b-9 in plasma and urine samples.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat C5b-9 neoantigen.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

complement C9

Cellular Localization

Secreted

Background

component of the terminal complement complex C5b-9, which induces cleavage and activation of caspase 3 and mediates induction of apoptosis [RGD, Feb 2006]

Database

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 399