Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Rabbit
Immunogen
Purified rabbit dihydropyridine receptor.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
ICC/IF analysis of PC-12 cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4ºC
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GTX22864 FACS Image
FACS analysis of SH-SY5Y cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue). Dilution : 1:100 for 60 min at room temperature Fixation : 2% paraformaldehyde
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GTX22864 IHC-P Image
IHC-P analysis of human skeletal muscle tissue using GTX22864 CACNA2D1 antibody [20A]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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GTX22864 ICC/IF Image
ICC/IF analysis of U251 cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 ºC
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GTX22864 FACS Image
FACS analysis of Neuro-2a cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue). Dilution : 1:100 for 60 min at room temperature Fixation : 2% paraformaldehyde
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GTX22864 WB Image
WB analysis of 25 ug of U87-MG (lane 1) and mouse brain (lane 2) cell lysates using GTX22864 CACNA2D1 antibody [20A]. Dilution : 1:1000
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GTX22864 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 ºC
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GTX22864 IHC-P Image
IHC-P analysis of mouse skeletal muscle tissue using GTX22864 CACNA2D1 antibody [20A]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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GTX22864 FACS Image
FACS analysis of C6 cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue). Dilution : 1:100 for 60 min at room temperature Fixation : 2% paraformaldehyde