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CAPG antibody [N3C3]

KO/KD
Anti-CAPG antibody [N3C3] used in Western Blot (WB). GTX114301
Anti-CAPG antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114301
Anti-CAPG antibody [N3C3] used in Western Blot (WB). GTX114301
Anti-CAPG antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114301

Cat. No. GTX114301

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CAPG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

NIH-3T3 , HeLa

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CAPG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619926

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

capping actin protein, gelsolin like , AFCP , HEL-S-66 , MCP

Cellular Localization

Cytoplasm , Nucleus , Melanosome

Background

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CAPG antibody [N3C3] used in Western Blot (WB). GTX114301

GTX114301 WB Image

KO/KD

Wild-type (WT) and CAPG knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CAPG antibody [N3C3] (GTX114301) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CAPG antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114301

GTX114301 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CAPG(GTX114301) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CAPG antibody [N3C3] used in Western Blot (WB). GTX114301

GTX114301 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX114301 diluted at 1:2000

Anti-CAPG antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114301

GTX114301 ICC/IF Image

CAPG antibody [N3C3] detects CAPG protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CAPG protein stained by CAPG antibody [N3C3] (GTX114301) diluted at 1:400.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Ohkawa Y et al. J Biol Chem 2023; 299 (8) : 105052 Involvement of langerin in the protective function of a keratan sulfate-based disaccharide in an emphysema mouse?model.
Wu Q et al. Discov Oncol 2023; 14 (1) : 69 Comprehensive analysis of ferroptosis-related genes for clinical and biological significance in hepatocellular carcinoma.
Tsung-Jung Tsai et al. Analytical Cellular Pathology 2018; 2018 : Article ID 8623937 Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration.
Application : WB, IHC-P
Reactivity : Human
Tsai TJ et al. Anticancer Res 2018; 38 (7) : 3943-3950 Gelsolin-like Actin-capping Protein (CapG) Overexpression in the Cytoplasm of Human Hepatocellular Carcinoma, Associated with Cellular Invasion, Migration and Tumor Prognosis.
Application : WB, IHC-P
Reactivity : Human
Tsai T.J. et al. hindawi 2018; (Epub) Capping actin protein (CapG) overexpression in human colorectal carcinoma and is contributed on tumor migration.
Application : WB, IHC-P
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169