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CAPZB antibody, C-term

Anti-CAPZB antibody, C-term used in Western Blot (WB). GTX89785
Anti-CAPZB antibody, C-term used in Western Blot (WB). GTX89785

Cat. No. GTX89785

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μg ($399)
See all CAPZB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89785-PEP).

Product Note

This antibody is expected to be specific for the beta2 isoform of capping protein, found in non-muscle cells and should not recognise the beta1 form.

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence NDLVEALKRKQQC, from the C Terminus of the protein sequence according to NP_004921.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

capping actin protein of muscle Z-line subunit beta , CAPB , CAPPB , CAPZ

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes the beta subunit of the barbed-end actin binding protein, which belongs to the F-actin capping protein family. The capping protein is a heterodimeric actin capping protein that blocks actin filament assembly and disassembly at the fast growing (barbed) filament ends and functions in regulating actin filament dynamics as well as in stabilizing actin filament lengths in muscle and nonmuscle cells. A pseudogene of this gene is located on the long arm of chromosome 2. Multiple alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-CAPZB antibody, C-term used in Western Blot (WB). GTX89785

GTX89785 WB Image

WB analysis of mouse (A), rat (B) and pig (C) heart lysates using GTX89785 CAPZB antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-CAPZB antibody, C-term used in Western Blot (WB). GTX89785

GTX89785 WB Image

WB analysis of Jurkat (A), HeLa (B) and NIH-3T3 (C) lysates using GTX89785 CAPZB antibody, C-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Gonz?lez-Barriga A et al. Front Cell Neurosci 2021; 15 : 662035 Integrative Cell Type-Specific Multi-Omics Approaches Reveal Impaired Programs of Glial Cell Differentiation in Mouse Culture Models of DM1.
Application : WB, ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399