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CARD11 antibody

Anti-CARD11 antibody used in IHC (Paraffin sections) (IHC-P). GTX55548
Anti-CARD11 antibody used in Western Blot (WB). GTX55548
Anti-CARD11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55548

Cat. No. GTX55548

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all CARD11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 985-1154 of human CARD11 (NP_115791.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase recruitment domain family member 11 , BENTA , BIMP3 , CARMA1 , IMD11 , IMD11A , PPBL

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CARD11 antibody used in IHC (Paraffin sections) (IHC-P). GTX55548

GTX55548 IHC-P Image

IHC-P analysis of human liver damage tissue using GTX55548 CARD11 antibody.
Dilution : 1:100

Anti-CARD11 antibody used in Western Blot (WB). GTX55548

GTX55548 WB Image

WB analysis of various sample lysates using GTX55548 CARD11 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CARD11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55548

GTX55548 ICC/IF Image

ICC/IF analysis of A549 cells using GTX55548 CARD11 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399