APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human C-CBL expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Cbl proto-oncogene , C-CBL , CBL2 , FRA11B , NSLL , RNF55
Cellular Localization
Cytoplasm,Cell membrane,Golgi apparatus
Background
This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Mar 2012]
Database
Research Area
DATA IMAGES
![Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481](/templates/default/js/lightbox/images/loading.gif "Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481")
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GTX60481 IHC-P Image
IHC-P analysis of ovarian cancer tissue using GTX60481 CBL antibody [3B12].
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GTX60481 WB Image
WB analysis of Raji (1), Raw264.7 (2),K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate using GTX60481 CBL antibody [3B12].
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GTX60481 IHC-P Image
IHC-P analysis of bladder cancer tissue using GTX60481 CBL antibody [3B12].
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GTX60481 FACS Image
FACS analysis of MCF-7 cells using GTX60481 CBL antibody [3B12].
Blue : CBL
Red : negative control
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GTX60481 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60481 CBL antibody [3B12].
Green : CBL
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60481 ELISA Image
ELISA analysis of antigen using GTX60481 CBL antibody [3B12].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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REFERENCE
There are currently no references for CBL antibody [3B12] (GTX60481). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CBL antibody [3B12] (GTX60481). Be the first to share your experience with this product.
![Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481](/upload/website/prouct_img/small/GTX60481/GTX60481_20170912_IHC-P_1_w_23061123_354.webp "Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481")
![Anti-CBL antibody [3B12] used in Western Blot (WB). GTX60481](/upload/website/prouct_img/small/GTX60481/GTX60481_20170912_WB_w_23061123_822.webp "Anti-CBL antibody [3B12] used in Western Blot (WB). GTX60481")
![Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481](/upload/website/prouct_img/small/GTX60481/GTX60481_20170912_IHC-P_w_23061123_615.webp "Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481")
![Anti-CBL antibody [3B12] used in Flow cytometry (FACS). GTX60481](/upload/website/prouct_img/small/GTX60481/GTX60481_20170912_FACS_w_23061123_742.webp "Anti-CBL antibody [3B12] used in Flow cytometry (FACS). GTX60481")
![Anti-CBL antibody [3B12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60481](/upload/website/prouct_img/small/GTX60481/GTX60481_20170912_ICCIF_w_23061123_690.webp "Anti-CBL antibody [3B12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60481")
![Anti-CBL antibody [3B12] used in ELISA (ELISA). GTX60481](/upload/website/prouct_img/small/GTX60481/GTX60481_20170912_ELISA_w_23061123_261.webp "Anti-CBL antibody [3B12] used in ELISA (ELISA). GTX60481")
![Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481](/upload/website/prouct_img/normal/GTX60481/GTX60481_20170912_IHC-P_1_w_23061123_354.webp "Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481")
![Anti-CBL antibody [3B12] used in Western Blot (WB). GTX60481](/upload/website/prouct_img/normal/GTX60481/GTX60481_20170912_WB_w_23061123_822.webp "Anti-CBL antibody [3B12] used in Western Blot (WB). GTX60481")
![Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481](/upload/website/prouct_img/normal/GTX60481/GTX60481_20170912_IHC-P_w_23061123_615.webp "Anti-CBL antibody [3B12] used in IHC (Paraffin sections) (IHC-P). GTX60481")
![Anti-CBL antibody [3B12] used in Flow cytometry (FACS). GTX60481](/upload/website/prouct_img/normal/GTX60481/GTX60481_20170912_FACS_w_23061123_742.webp "Anti-CBL antibody [3B12] used in Flow cytometry (FACS). GTX60481")
![Anti-CBL antibody [3B12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60481](/upload/website/prouct_img/normal/GTX60481/GTX60481_20170912_ICCIF_w_23061123_690.webp "Anti-CBL antibody [3B12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60481")
![Anti-CBL antibody [3B12] used in ELISA (ELISA). GTX60481](/upload/website/prouct_img/normal/GTX60481/GTX60481_20170912_ELISA_w_23061123_261.webp "Anti-CBL antibody [3B12] used in ELISA (ELISA). GTX60481")