APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 2-3μg/ml |
|
Note :
IHC-P
Human Placenta shows strong nuclear staining in trophoblasts.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89455-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QSQKTAHETDKSE, from the internal region of the protein sequence according to NP_006798.1; NP_001120700.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
chromobox 1 , CBX , HP1-BETA , HP1Hs-beta , HP1Hsbeta , M31 , MOD1 , p25beta
Cellular Localization
Nucleus
Background
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89455 WB Image
WB analysis of A431 lysate using GTX89455 CBX1 / HP1 beta antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89455 IHC-P Image
IHC-P analysis of human placenta using GTX89455 CBX1 / HP1 beta antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
|
REFERENCE
There are currently no references for CBX1 / HP1 beta antibody, Internal (GTX89455). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CBX1 / HP1 beta antibody, Internal (GTX89455). Be the first to share your experience with this product.
