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CD11b antibody [ICRF44] (Azide free)

Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296
Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296
Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296
Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296
Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296
Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296
Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296
Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296

Cat. No. GTX76296

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 ICRF44

Isotype

 IgG1

Application

 IHC-Fr, FACS, IP, Functional Assay

Reactivity

 Human, Baboon, Cynomolgus monkey, Rhesus Monkey
Package
200 μg ($399)
See all CD11b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr 1/50-1/500
FACS 1/50-1/100
IP Assay dependent
Functional Assay Assay dependent

Note :

IHC-Fr
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rheumatoid synovial cells and human monocytes.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_377263

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha M , CD11B , CR3A , MAC-1 , MAC1A , MO1A , SLEB6

Cellular Localization

Cell membrane

Background

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296

GTX76296 IHC-Fr Image

IHC-Fr analysis of human spleen tissue using GTX76296 CD11b antibody [ICRF44] (Azide free).

Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296

GTX76296 IHC-Fr Image

IHC-Fr analysis of human spleen tissue using GTX76296 CD11b antibody [ICRF44] (Azide free).

Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296

GTX76296 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX76296 CD11b antibody [ICRF44] (Azide free).

Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296

GTX76296 IHC-Fr Image

IHC-Fr analysis of mouse lymph node using CD11b antibody [ICRF44] (Red), CD21 antibody [YTS105.18] (Green, GTX74728) and DAPI (blue).

Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296

GTX76296 IHC-Fr Image

IHC-Fr analysis of mouse lymph node using CD11b antibody [ICRF44] (Red), CD21 antibody [YTS105.18] (Green, GTX74728) and DAPI (blue).

Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296

GTX76296 IHC-Fr Image

IHC-Fr analysis of mouse lymph node using CD11b antibody [ICRF44] (Red), CD21 antibody [YTS105.18] (Green, GTX74728) and DAPI (blue).

Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296

GTX76296 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX76296 CD11b antibody [ICRF44] (Azide free).

Anti-CD11b antibody [ICRF44] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX76296

GTX76296 IHC-Fr Image

IHC-Fr analysis of human spleen tissue using GTX76296 CD11b antibody [ICRF44] (Azide free).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations