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CD11b antibody [M1/70.15] (Biotin)

Anti-CD11b antibody [M1/70.15] (Biotin) used in Flow cytometry (FACS). GTX76469
Anti-CD11b antibody [M1/70.15] (Biotin) used in Flow cytometry (FACS). GTX76469

Cat. No. GTX76469

Reference 5

Host

 Rat

Clonality

 Monoclonal

Clone Name

 M1/70.15

Isotype

 IgG2b

Application

 FACS

Reactivity

 Mouse
Package
100 μg ($339)
See all CD11b products

APPLICATION

Application Note

For FACS: Use at 0.2 ug to label 10^6 cells. Protocols are available upon request. Optimal dilutions/concentrations should be determined by the end user.

Product Note

Mouse CD11b

PROPERTIES

Form

Liquid

Buffer

PBS, BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

C57BL/10 spleen cell enriched for T lymphocytes

Purification

Protein G affinity purified

Conjugation

Biotin

RRID

AB_378546

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Integrin Alpha M , Cd11B/Cd18 , Cr3 , Cr3A , Cd11B , F730045J24Rik , Ly-40 , Mac1 , Mac-1 , Mac-1A , Itgam

Background

Recognises the murine CD11b cell surface antigen (also known as the alpha M integrin chain), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.

Research Area

DATA IMAGES

Anti-CD11b antibody [M1/70.15] (Biotin) used in Flow cytometry (FACS). GTX76469

GTX76469 FACS Image

Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE (GTX76475)

Anti-CD11b antibody [M1/70.15] (Biotin) used in Flow cytometry (FACS). GTX76469

GTX76469 FACS Image

Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b (GTX76473)

REFERENCE

Clone Reference

Koutarou Nakamura et al. PLoS Biol 2021; 19 (5):e3000939 Extracellular DJ-1 induces sterile inflammation in the ischemic brain.
Wang HT et al. J Food Drug Anal 2018; 26 (2):678-687 Characteristics of fucose-containing polysaccharides from submerged fermentation of?Agaricus blazei Murill.
Zhang Z et al. Neurobiol Dis 2017; 111:153-162 Trajectory of inflammatory and microglial activation markers in the postnatal rabbit brain following intrauterine endotoxin exposure.
Wang HT et al. Journal of Food and Drug Analysis 2017; (Epub) Characteristics of fucose-containing polysaccharides from submerged fermentation of Agaricus blazei Murill.
Yang LC et al. Evid Based Complement Alternat Med 2013; 2013:458075 a type ii arabinogalactan from anoectochilus formosanus for g-csf production in macrophages and leukopenia improvement in ct26-bearing mice treated with 5-*.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339