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CD130 antibody

Anti-CD130 antibody used in IHC (Paraffin sections) (IHC-P). GTX109048
Anti-CD130 antibody used in IHC (Paraffin sections) (IHC-P). GTX109048
Anti-CD130 antibody used in Western Blot (WB). GTX109048

Cat. No. GTX109048

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all CD130 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Mouse, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human CD130. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729646

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 6 signal transducer , CD130 , CDW130 , GP130 , IL-6RB

Cellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD130 antibody used in IHC (Paraffin sections) (IHC-P). GTX109048

GTX109048 IHC-P Image

CD130 antibody detects CD130 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CD130 stained by CD130 antibody (GTX109048) diluted at 1:7500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD130 antibody used in IHC (Paraffin sections) (IHC-P). GTX109048

GTX109048 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD130(GTX109048) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD130 antibody used in Western Blot (WB). GTX109048

GTX109048 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD130 antibody (GTX109048) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ma B et al. Exp Eye Res 2018; (Epub) Effects of Interleukin-6 on posterior capsular opacification.
Reactivity : Rat
Pathare AD et al. Am J Reprod Immunol 2017; (Epub) Downregulation of genes related to immune and inflammatory response in IVF implantation failure cases under controlled ovarian stimulation.
Application : IHC-P
Reactivity : Human
Zhang Y et al. J Cancer Res Clin Oncol 2014; 140 (2) : 329-40 Tumor self-seeding by circulating tumor cells in nude mouse models of human osteosarcoma and a preliminary study of its mechanisms.
Application : WB, ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169