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CD133 antibody [6H10-F1-C11]

Anti-CD133 antibody [6H10-F1-C11] used in Western Blot (WB). GTX49150
Anti-CD133 antibody [6H10-F1-C11] used in Western Blot (WB). GTX49150

Cat. No. GTX49150

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6H10-F1-C11

Isotype

 IgG2a

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all CD133 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human PROM1 protein fragments expressed in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prominin 1 , AC133 , CD133 , CORD12 , MCDR2 , MSTP061 , PROML1 , RP41 , STGD4

Cellular Localization

Apical cell membrane , Endoplasmic reticulum

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CD133 antibody [6H10-F1-C11] used in Western Blot (WB). GTX49150

GTX49150 WB Image

WB analysis of CaCo2 lysate using Prominin-1 antibody at a dilution of 1:1,000.

Anti-CD133 antibody [6H10-F1-C11] used in Western Blot (WB). GTX49150

GTX49150 WB Image

WB analysis of lysates from (A) non-transfected and (B) PROM1-transfected CHO-k1 cells using Prominin-1 antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399