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CD133 antibody

Anti-CD133 antibody used in Western Blot (WB). GTX32496
Anti-CD133 antibody used in IHC (Paraffin sections) (IHC-P). GTX32496
Anti-CD133 antibody used in Immunoprecipitation (IP). GTX32496
Anti-CD133 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32496
Anti-CD133 antibody used in IHC (Paraffin sections) (IHC-P). GTX32496

Cat. No. GTX32496

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CD133 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 170-430 of human PROM1 (NP_001139320.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887659

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prominin 1 , AC133 , CD133 , CORD12 , MCDR2 , MSTP061 , PROML1 , RP41 , STGD4

Cellular Localization

Apical cell membrane,Endoplasmic reticulum

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CD133 antibody used in Western Blot (WB). GTX32496

GTX32496 WB Image

WB analysis of various sample lysates using GTX32496 CD133 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CD133 antibody used in IHC (Paraffin sections) (IHC-P). GTX32496

GTX32496 IHC-P Image

IHC-P analysis of human lung tissue using GTX32496 CD133 antibody.
Dilution : 1:100

Anti-CD133 antibody used in Immunoprecipitation (IP). GTX32496

GTX32496 IP Image

IP analysis of HT-29 cell lysate using GTX32496 CD133 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-CD133 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32496

GTX32496 ICC/IF Image

ICC/IF analysis of C6 cells using GTX32496 CD133 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD133 antibody used in IHC (Paraffin sections) (IHC-P). GTX32496

GTX32496 IHC-P Image

IHC-P analysis of human prostate tissue using GTX32496 CD133 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Bhattacharya S et al. Invest Ophthalmol Vis Sci 2017; 58 (4) : 2366-2387 Prominin-1 Is a Novel Regulator of Autophagy in the Human Retinal Pigment Epithelium.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399