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CD14 antibody [UCHM1] (FITC)

Anti-CD14 antibody [UCHM1] (FITC) used in Flow cytometry (FACS). GTX76340

Cat. No. GTX76340

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 UCHM1

Isotype

 IgG2a

Application

 FACS

Reactivity

 Human, Cynomolgus monkey, Rhesus Monkey, Trout
Package
100 μg ($399)
See all CD14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Neat - 1/10

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Product Note

This antibody reacts with Kupffer cells and sinus lining cells on the liver. Skin  Langerhans  cells  are  always  negative   (Hogg et al. 1984).

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Thymocytes followed by peripheral blood mononuclear cells.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_377294

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD14 molecule

Cellular Localization

Cell membrane , Secreted , Golgi apparatus

Background

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-CD14 antibody [UCHM1] (FITC) used in Flow cytometry (FACS). GTX76340

GTX76340 FACS Image

Figure A. RPE conjugated Mouse anti Human CD9 (GTX76186) and FITC conjugated Mouse IgG2a isotype control (GTX30858). Figure B. RPE conjugated Mouse anti Human CD9 (GTX76186) and FITC conjugated Mouse anti Human CD14 (GTX76340). All experiments performed on human Peripheral blood mononuclear cells

REFERENCE

Clone Reference

Meppelink AM et al. Cytotherapy 2016; 18 (6):729-39 Rapid isolation of bone marrow mesenchymal stromal cells using integrated centrifuge-based technology.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399