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CD14 antibody

Anti-CD14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32497
Anti-CD14 antibody used in IHC (Paraffin sections) (IHC-P). GTX32497
Anti-CD14 antibody used in Western Blot (WB). GTX32497
Anti-CD14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32497

Cat. No. GTX32497

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all CD14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CD14 (NP_001167576.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD14 molecule

Cellular Localization

Cell membrane,Secreted,Golgi apparatus

Background

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-CD14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32497

GTX32497 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX32497 CD14 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD14 antibody used in IHC (Paraffin sections) (IHC-P). GTX32497

GTX32497 IHC-P Image

IHC-P analysis of human spleen tissue using GTX32497 CD14 antibody.
Dilution : 1:100

Anti-CD14 antibody used in Western Blot (WB). GTX32497

GTX32497 WB Image

WB analysis of various sample lysates using GTX32497 CD14 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CD14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32497

GTX32497 ICC/IF Image

ICC/IF analysis of Raw264.7 cells using GTX32497 CD14 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399