Host |
Mouse |
---|---|
Clonality |
Monoclonal |
Clone Name |
AZN-L50 |
Isotype |
IgG2a |
Application |
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, Neutralizing/Inhibition |
Reactivity |
Human |
Package |
250 μg ($699)
|
Application | Recommended Dilution |
---|---|
WB | Assay dependent |
ICC/IF | 4 μg/ml |
IHC-P | Assay dependent |
IHC-Fr | Assay dependent |
FACS | 4μg/ml antibody / 2x10⁵ K562 cells |
IP | Assay dependent |
Neutralizing/Inhibition | Assay dependent |
Note : WBA non-reduced sample treatment and 6.5 % SDS-Page was used. ICC/IF Antibody AZN-L50 was used on methanol/acetone fixed monolayers of cells grown on a glass surface. IHC-P Tissue sections were pretreated by heating in a rice steamer in citrate buffer for 10 minutes for antigen retrieval. Tissue sections were blocked with normal horse serum. IHC-Fr Tissue sections were fixed in acetone IP Radioactive labelled KG1a cells were lysed and precipitated with 1 μg of antibody coupled to Protein G Sepharose 4 Fast Flow beads. Neutralizing/Inhibition Antibody AZN-L50 functions as an blocking antibody for homotypic ALCAM-ALCAM interaction. |
Package | List Price ($) |
---|---|
$ 699 | |
Custom Conjugations |