CD23 antibody [EBVCS-5] (APC)

Cat. No. GTX01482-07

Host	Mouse
Clonality	Monoclonal
Clone Name	EBVCS-5
Isotype	IgG1
Application	FACS
Reactivity	Human
Package	100 test ($349)

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View product citations for antibody GTX01482-07 on CiteAb

PRODUCT

Summary

The EBVCS-5 antibody is specific for human CD23, also known as the low affinity IgE receptor (FceRII), a 45 kDa type II transmembrane protein and member of the immunoglobulin superfamily. It is expressed on most B cells and is upregulated upon activation. It is also present on mantle zone B cells, eosinophils, monocytes and a subset of T cells and platelets. CD23 plays a role in B cell development and differentiation, and also functions to regulate IgE production. Soluble secreted forms of CD23 have been reported to bind IgE, stimulate release of pro-inflammatory cytokines from monocytes, and play a role in B cell differentiation.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	0.125 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.025 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fc fragment of IgE receptor II , BLAST2 , CD23 , CD23A , CLEC4J , FCE2 , FCER2 , IGEBF

Cellular Localization

Cell membrane,Secreted

Background

The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Database

Gene ID: 2208 FCER2
UniProt: P06734 FCER2

Research Area

DATA IMAGES

GTX01482-07 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01482-07 CD23 antibody [EBVCS-5] (APC).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.125 μg (5 μl)