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CD26 antibody [11D7]

Overexpression
Anti-CD26 antibody [11D7] used in Flow cytometry (FACS). GTX84602
Anti-CD26 antibody [11D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84602
Anti-CD26 antibody [11D7] used in Western Blot (WB). GTX84602
Anti-CD26 antibody [11D7] used in IHC (Paraffin sections) (IHC-P). GTX84602

Cat. No. GTX84602

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 11D7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($539)
See all CD26 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
ELISA Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human DPP4 (NP_001926) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10731723

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dipeptidyl peptidase 4 , ADABP , ADCP2 , CD26 , DPPIV , TP103

Cellular Localization

Apical cell membrane,Cell junction

Background

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.

Database

Research Area

DATA IMAGES

Anti-CD26 antibody [11D7] used in Flow cytometry (FACS). GTX84602

GTX84602 FACS Image

FACS analysis of HeLa cells using GTX84602 CD26 antibody [11D7].
Red : Primary antibody
Blue : Negative control antibody

Anti-CD26 antibody [11D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84602

GTX84602 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CD26 plasmid using GTX84602 CD26 antibody [11D7].

Anti-CD26 antibody [11D7] used in Western Blot (WB). GTX84602

GTX84602 WB Image

Overexpression

WB analysis of HEK293T cells transfected with CD26 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84602 CD26 antibody [11D7].
Loading : 5 ug per lane

Anti-CD26 antibody [11D7] used in IHC (Paraffin sections) (IHC-P). GTX84602

GTX84602 IHC-P Image

IHC-P analysis of liver tissue using GTX84602 CD26 antibody [11D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

Application Reference

Lai HC et al. Int J Mol Sci 2023; 24 (22) Dipeptidyl Peptidase 4 Stimulation Induces Adipogenesis-Related Gene Expression of Adipose Stromal Cells.
Chen LW et al. Stem Cell Res Ther 2022; 13 (1) : 357 Adipose-derived stromal cells reverse insulin resistance through inhibition of M1 expression in a type 2 diabetes mellitus mouse model.
Chen LW et al. PLoS One 2021; 16 (5) : e0252153 Inhibiting adipose tissue M1 cytokine expression decreases DPP4 activity and insulin resistance in a type 2 diabetes mellitus mouse model.
Application : WB
Reactivity : Mouse
Okondo MC et al. Nat Chem Biol 2016; (Epub) DPP8 and DPP9 inhibition induces pro-caspase-1-dependent monocyte and macrophage pyroptosis.
Application : ELISA
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539