GeneTex

  • Country / Location Selection

United States (US)

CD3 epsilon antibody [APA1/1] (PE)

Anti-CD3 epsilon antibody [APA1/1] (PE) used in Flow cytometry (FACS). GTX80237

Cat. No. GTX80237

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 APA1/1

Isotype

 IgG1

Application

 FACS

Reactivity

 Human, Mouse
Package
100 μg ($339)
See all CD3 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Product Note

This antibody recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human CD3 proteins isolated from thymus

Purification

Purified by size-exclusion chromatography

Conjugation

Phycoerythrin (PE)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3E , CD3e molecule , IMD18 , T3E , TCRE , CD3 epsilon

Cellular Localization

Membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 epsilon antibody [APA1/1] (PE) used in Flow cytometry (FACS). GTX80237

GTX80237 FACS Image

FACS analysis of Jurkat cells using GTX80237 CD3 (activation epitope) antibody [APA1/1] (PE)

REFERENCE

There are currently no references for CD3 epsilon antibody [APA1/1] (PE) (GTX80237). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for CD3 epsilon antibody [APA1/1] (PE) (GTX80237). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339